Sentence examples for using a nuclear extraction from inspiring English sources

Nuclear extracts were prepared using a nuclear extraction kit from Active Motif (Belgium, UK) according to manufacturer's instructions.

Nuclear proteins were extracted from the HRGECs using a nuclear extraction kit (Pierce Biotechnology, Rockford, Illinois, USA) and incubated with a biotin-labeled NF-κB probe (5′-AGT TGA GGG GAC TTT CCC AGG C-3′) at room temperature for 20 min.

ARPE-19 cell nuclear extracts were prepared using a nuclear extraction kit (Millipore) following the manufacturer's protocol.

Cytoplasmic and nuclear extracts were prepared using a nuclear extraction kit (Chemicon, Temecula, CA) according to the manufacturer's instructions and our published methods [ 18, 19].

Nuclear extracts were prepared from treated and control MHCC97H cells using a nuclear extraction kit (Active Motif).

Qualitative western blot analyses Nuclear and cytoplasmic extracts of liver and pancreas were prepared using a nuclear extraction Kit (Chemicon).

The cells were rinsed twice with phosphate-buffered saline and nuclear extracts were isolated using a nuclear protein extraction kit (Chemicon, Temecula, CA, USA) and protein estimation was performed using the micro-BCA kit (Pierce, Rockford, IL, USA).

Total proteins were obtained from human cartilage samples and chondrocyte cultures using radioimmunoprecipitation assay (RIPA) lysis buffer (Biyotime), while nuclear proteins were extracted using a nuclear protein extraction kit (Biyotime).

In brief, nuclear proteins were isolated from epithelial cells using a nuclear protein extraction kit (Active Motif).

Specifically, nuclear and cytoplasmic protein was extracted using a nuclear and cytoplasmic protein extraction kit (P0028, Beyotime biotechnology, Shanghai, China).

The cytosol and nuclear protein were isolated using a nuclear and cytoplasmic protein extraction kit (Sangon Biotech, Shanghai, China) according to the manufacturer's protocol.