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Control ChIP was performed using a normal rabbit IgG (Santa Cruz, Santa Cruz, CA).
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This study aimed to define efficient and safe tissue-selective targeted gene therapy approaches for delivering genes into keratocytes of the cornea in vivo using a normal or diseased rabbit model.
The acoustic behavior of the nanosized contrast agent was evaluated using power Doppler imaging in a normal rabbit model.
An anti-ATF2 antibody was used to pull down the complex from these lysates; a normal rabbit IgG was used as a negative control.
An affinity isolated antibody, a buffered aqueous solution of biotinlyated antirabbit IgG antibody (whole-molecule), was produced in goat; rabbit IgG purified from a normal rabbit serum by using fractionation and ion-exchange chromatography; phosphate-buffered saline (PBS); dimethyl sulfoxide (DMSO); and 3,3′,5,5′-tetramethybezidine (TMB) were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
A normal rabbit serum was used as a negative control.
Serum blocking was performed using 10% normal rabbit serum for 30 min at room temperature.
Nonspecific binding was blocked by using 10% normal rabbit serum and 0.5%.
A negative control to each section was prepared by using normal rabbit serum instead of the primary antibody.
The DNA levels were normalized to those of the appropriate chromatin inputs after they are subtracted with DNA signals given by ChIP using normal rabbit or goat IgG.
Granta 519 cells were cross-linked, and protein-DNA complexes were immunoprecipitated using antibodies recognizing normal rabbit IgG or anti-SOX11 antibody.
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