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Dynamic improvement of peak capacity in EFGF can be achieved by using a nonlinear gradient.
Afterwards, this fraction was eluted using a nonlinear gradient with buffer A (0.1% trifluoroacetic acid [TFA] in Milli-Q water) and buffer B (66% acetonitrile in buffer A).
TTR was eluted from the column using a nonlinear gradient at a flow rate of 0.6 mL/min over 33 min (see the Supporting Information for additional details).
Afterwards, this fraction was eluted by using a nonlinear gradient with buffer A (0.1% of trifluoroacetic acid in Milli-Q water) and buffer B (acetonitrile 66% in buffer A).
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The metabolites in the urine extract were chromatographically resolved by high-performance liquid chromatography (HPLC) using a Surveyor HPLC system (ThermoFinnigan, San Jose, CA) equipped with a Betasil phenyl HPLC column (3 μm, 100 mm × 2.1 mm; ThermoHypersil-Keystone, Bellefonte, PA) using a nonlinear water:acetonitrile solvent gradient.
Grainger and Lee [16, 17, 18] formulated the problem using a nonlinear programming model and solved it by simple iterative procedures based on gradient search.
Concentration response relationships were fit using a nonlinear regression.
IC50 values were calculated using a nonlinear regression curve fit.
Population pharmacodynamics was modeled using a nonlinear mixedeffect model.
The ModEM algorithm is a 3D inversion algorithm that uses a nonlinear conjugate gradient (NLCG) scheme for the parameter search.
We notice that the use of the ohmic representation yields a nonlinear gradient form of the DD current density.
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