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The maximum power value of each laser source was reduced to approximately 0.4 mW at the sample using a neutral density filter.
A further reduction of the incident intensity using a neutral density filter in front of the laser source revealed that the time constants were independent of the incident intensity.
The system employs a HeNe laser (632.8 nm, JDSU, 1 mW) attenuated using a neutral density filter, and focused into a fibre-optic cable for delivery to a Horiba Superhead sampling accessory.
The same procedure was subsequently applied to acquire the excitation images using a neutral density filter to avoid CCD saturation (integration time 0.1 s).
The light intensity was adjusted to 1 μmol quanta m-2 s-1 for all three light spectra using a neutral density filter (Lee filter model, neutral density 210 (0.6) 20% transmission) when needed.
The scattering beam from the sample plane is collected by a condenser lens and reduced in the intensity using a neutral density filter and measured by an avalanche photodiodes (Hamamatsu, G8931-04) monnted on a manual stage.
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You can skip this step if you used a neutral density filter on the sky.
Using a neutral category.
The laser power delivered to the eye lens was controlled using a graded neutral density attenuation filter.
The fiber output power was adjusted using a variable neutral density (ND) filter wheel appropriately placed in the beam path.
Ilp7 or Va neurons were recorded with a 100 ms exposure time in 0.5 s intervals on an inverted compound microscope (Axiovert, Zeiss), using a 2.0 neutral density filter, a GFP/DsRed emission filter set (Chroma), a 40x C-Apochromat lens (Zeiss), a Dual-View beamsplitter with GFP/DsRed emission filters (Photometrics) and a Cascade II 512 EMCCD camera (Photometrics).
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using a neutral vegetable
using a neutral middle
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using a neutral background
using a neutral vocabulary
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using a neutral condition
using a current density
using a neutral alumina
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using a simplified density
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