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This method incorporates several essential elements to make it both sensitive and specific, including a "blockerette" linker, magnetic capture of target sequences, and exponential replication of potentially rare sequences using a nested promoter for T7 RNA polymerase, followed by nested RT-PCR.
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Using a nested design without temporal replication, replicate core samples were taken from sampling stations nested within lagoons that were nested within catchment type.
In order to estimate the spectral densities for the entire sample, we nest this model using a nested DP process.
Env was amplified using a nested PCR.
The FCGR2B-1206G/A polymorphism was genotyped using a nested RFLP assay.
Design Retrospective cohort study using a nested case-control analysis.
This was done using a nested anova, with lake as a fixed factor nested within perturbation category.
We used a nested case-control design.
Each marker was reamplified using a nested-PCR approach.
The pairs seem to be able to build several nests sequentially or concomitantly before actively using a nest.
After all, everything from a bluebird to an Osprey can use a nest box and nest platform!
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