Using a nest of fine pipes that resemble a large wire coil, the engineers have managed to get round this fatal problem that would normally follow from such rapid cooling of the moisture in atmospheric air.
In my work on iMHG, using a nested case control study that was not cited by Fewtrell (2004), I confirmed that cofactors (feeding practices, individual and infant physiology, etc).
Samples were also tested at the ospC locus, using a nested-PCR protocol that produced an approximately 500-bp ospC fragment (47).
HAdV positive samples were further amplified using a nested PCR procedure that targeted hyper variable regions 1 6 of the hexon gene as described by Lu and Erdman [ 21].
KIPyV detection was performed by using a nested PCR approach that targeted the VP1 capsid gene as described by Allander et al. (1 ).
The DNA extract from each tick was tested for A. phagocytophilum by using a nested PCR assay that amplified a 456-bp portion of the 5′ region of the 16S rRNA gene as previously described (6 ).
This is in keeping with our findings that classical CCK2-R expression is undetectable in these cell lines by real-time PCR (data not shown) or using a nested PCR method that avoids potential artefacts associated with amplification of genomic DNA (Grabowska et al, 2004).
The methylation pattern within the CpG island in exon 1 of the CDH1 gene (sequence -126 bp to +144 bp relative to transcription start, GenBank accession number D49685) was determined using a nested-PCR approach that has been published previously [ 27].
A detailed study of method precision using a nested design demonstrated that variation sources such as instruments, operators and time did not interfere in the variability of results within laboratory and consequently in its intermediate precision.
We failed to replicate this finding using a nest-heating experiment that targeted a single parent and was carried out in the same study area, on the same species and using a similar incubation monitoring system.
