Sentence examples for using a negative selection from inspiring English sources

Interestingly, both isolated clones exhibited high selectivity for H1299 cells without using a negative selection step.

The first example considered in this section is using a negative selection clause, WHERE Age 22, so it is also an example where v-tables queries would not report sure information.

CD4 T cells were MACS purified using a negative selection kit from Miltenyi.

Mouse NK cells were isolated using a negative selection kit (NK cell isolation Kit mouse, 130-090-864; Miltenyi Biotec).

Lin- cells were isolated using a negative selection column according to the manufacturer's instructions (StemCell Technologies, Canada).

The samples were enriched for DCs using a negative selection enrichment cocktail (EasySep Human Pan-DC Pan-DC Pre-enrichment Pre-enrichment Kit) according to the manufacturer'StemCellol.

We investigated the ability of givinostat, belinostat (250 and 500 nM) and panobinostat (7.5 and 15 nM) to reactivate latent HIV-1 in a CCL19-induced latent primary CD4+ T cell infection model as described previously., Briefly, resting CD4+ T cells were isolated from buffy coat using a negative bead selection strategy.

It is based on pHW2000 (Hoffmann et al., 2000b) but contains additionally (i) the ccdB gene whose expression is lethal for most Escherichia coli strains and therefore used as a negative selection marker and (ii) more efficient AarI cloning sites that flank the ccdB gene on either side.

Coverage data can also be used in a negative selection algorithm to develop assays to detect novel microbes in tissue samples.

Superior models are ranked higher, and higher ranking models have an increasingly large probability of selection using a negative exponential distribution.

Thus, highly selective clone can be identified using this technique without the use of a negative selection step.