Sentence examples for using a negative isolation from inspiring English sources

Human peripheral B cells were isolated using a negative isolation kit (StemCell Technologies, Vancouver, British Columbia) according to manufacturer's instructions.

Responder CD4+ T cells used for allogeneic MLR assays were isolated from PBMC of healthy donors using a negative isolation kit (Dynal biotech-Invitrogen, France).

T cells were purified from PBMCs using a negative isolation kit from Miltenyi (Auburn, CA).

Otherwise, PB T cells were purified using a negative isolation procedure (T Cell Negative Isolation kit; Dynal; Invitrogen, Carlsbad, CA, USA), which resulted in a 95% CD3+ cell population, as assessed by FACS analysis after staining with a peridinin chlorophyl protein (PerCP -conjugated anti-CD3 monoclonal antibody (Becton Dickinson, Franklin Lakes, NJ, USA).

CD4+ T cells were isolated using a negative selection CD4+ isolation kit (Miltenyi Biotec).

Vehicle- or ZOL-treated monocytes (1 × 10 cells/well) were then co-cultured with the monocyte-depleted PBMCs (5 × 10 cells/well) or with γδ T cells (1 × 10 cells/well) isolated from the monocyte-depleted fraction using a negative γδ T cell isolation kit (Miltenyi Biotec GmbH), in a final volume of 500 μl medium in 24-well plates.

Mouse NK cells were isolated using a negative selection kit (NK cell isolation Kit mouse, 130-090-864; Miltenyi Biotec).

NK cells were isolated from PBMC by magnetic-activated cell sorting (MACS) using a negative selection kit (NK cell isolation kit II, Miltenyi Biotec, Bergisch-Gladbach, Germany) and either used directly or cultured for 4 days with 100 U/ml IL-2.

CD8+ T cells were isolated from PBMC by MACS using a negative selection kit (CD8+ T cell isolation kit, Miltenyi Biotec).

Then, PBM were isolated from PBMC using a monocyte negative isolation kit (Dynal Biotech Inc).

You should not use a negative approach.