Sentence examples for using a mutation system from inspiring English sources

Using a mutation system, they further identified the essential role of an internal loop within the miR-107::let-7 duplex, which provided important clues for further investigation on the underlying mechanism [ 35].

Electrophoresis was performed in a 1 × TAE buffer with a constant voltage of 80 V for 17 h at 60°C using a DCode Mutation Detection System (Bio-Rad, USA).

Various DNA analysis techniques such as dot blot analysis, reverse dot blot, allele specific amplification using amplification refractory mutation system (ARMS) or direct DNA sequencing have been widely used to identify beta-globin gene mutations.

Molecular HLA typing was performed on most study subjects using amplification refractory mutation system PCR (ARMS-PCR) with sequence specific primers, as previously described[28].

BRAF mutations were detected using Amplification Refractory Mutation System allele-specific PCR.

Recent advances in the functional analysis of mutations using a heterologous expression system and genetically engineered animal models have provided significant insights into the underlying molecular mechanisms responsible for inherited arrhythmia.

We identified mutations using a simple scoring system to penalize polymorphisms that were likely to be due to homopolymer sequencing errors, which are caused by 454 sequencing technology, or misassembly errors, which are caused by assembly algorithms (Table S4; http://genomevolution.org/paper_supp_data/8-Ecoli-genomes-2010/).

It is difficult to test stress-induced mutation experimentally using a system that shows some mutagenesis, even if the observed mutagenesis is neither necessary nor sufficient to explain the revertants.

Detection of Ha- ras codon 12 mutation was conducted using a commercial SNP system (ABI, USA) [ 41].

Detection of Ki- ras codon 12 mutation was conducted using a commercial SNP system following the manufacturer's instructions [ 42] (Roche, Germany).

High Resolution Melting Curve analysis for variant positions in RB1 exon 2 and MLL exon 32 mutations was performed using a LightCycler 480 system (Roche Diagnostics, Penzberg, Germany).