Sentence examples for using a multiplexed polymerase from inspiring English sources

We amplified and sequenced genomic DNA from the peripheral blood of 587 healthy volunteers using a multiplexed polymerase chain reaction assay that targets the variable region of the rearranged TCRβ locus, and we determined the presence and the proportion of productive rearrangements for each TCRβ V gene segment in each individual.

We propose to implement a modal wavefront sensor (MWS) using a multiplexed phase computer-generated hologram (MPCGH).

Levels of human cytokines in the culture supernatants of HIV-exposed B cells were quantified using a multiplexed (Luminex platform) immunometric assay (MILLIPLEX xMAP High Sensitivity Human Cytokine Panel, Millipore).

Eight proteins were verified using a multiplexed selected reaction monitoring assay, in an independent skin sample set.

Gene expression was analyzed using a multiplexed TaqMan assay on demand on the 7500 Fast Real-Time PCR system (Applied Biosystems).

Briefly, IDH1 and IDH2 mutational analyses were performed using a multiplexed SNaPshot® reaction and detection by capillary electrophoresis [ 25].

Samples were tested for 12 respiratory viruses by using a multiplexed panel of real-time, TaqMan reverse transcription PCRs.

In addition, multiple mutants can be analyzed simultaneously using a multiplexed setup with individually barcoded samples in single sequencing run.

Briefly, a multiplexed polymerase chain reaction (PCR) method was employed using a mixture of 60 forward primers specific to TCR Vβ gene segments and 13 reverse primers specific to TCR Jβ gene segments, and 87 base pair (bp) reads were obtained using the Illumina HiSeq System (Illumina Inc., San Diego, CA).

DNA was amplified in a multiplexed polymerase chain reaction (PCR) containing primers specific for the cry9Aa2 gene, the nptII gene, and the endogenous potato actin gene as an internal control (Table 1).

The system uses a multiplexed microarray-based technology.