High-risk human papillomavirus detection and genotyping were done using a multiplex test based on the Luminex technology (Multimetrix, Heidelberg, Germany) allowing for standardised detection and typing of the high-risk types HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73, and 82.
A major consideration when deciding between a targeted, single-gene approach to genetic testing versus testing using a multiplex gene panel is the increased chance of receiving inconclusive results, which presents challenges for both patients and clinicians.
The 14 polymorphic markers (Table 1) were selected for further testing using a multiplex PCR set-up with 304 S. acaulis individuals collected in Endalen and Hotellneset (Svalbard, Norway).
The extracted nucleic acid was tested using a multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay.
Using a multiplex serological assay, 18 we tested for a wide range of β human papillomaviruses in plasma samples collected as part of a large population based case-control study.
Among 84 genes tested by using a multiplex real-time PCR Cancer Pathway Array approach, 34 and 16, respectively, were differentially expressed between OvCar-3 and PC-3 OPNc-overexpressing cells in relation to control clones.
Both KRAS (codons 12 and 13) and BRAF (p.V600E) mutation tests were performed using a multiplex allele-specific PCR-based assay (ACCB, Beijing, China), together with the Stratagene Mx3000P (Agilent Technologies Inc ,Santa Clara, CA), which assesses seven different potential mutations in KRAS codons 12 and 13 (Gly12Asp, Gly12Arg, Gly12Cys, Gly12Ser, Gly12Val, Gly12Vand and Gly13Asp).
Isolates that were mecA-negative but resistant to ceftiofur were also tested for the novel mec homologue mecC using a multiplex PCR [ 16].
Using a multiplex PCR assay, X. oryzae pv.
Genotyping was performed using a multiplex protocol (see Supporting Information).
