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To investigate the relation between functional impairments of cancer patients and circulating cytokines using a multiplex technique.
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We evaluated the GSTM1 and GSTT1 genetic polymorphisms using a multiplex PCR technique, previously described by our laboratory (Liu et al. 2001).
Screening of the qnrA-, qnrB-, and qnrS genes in water samples and from isolated colonies was conducted by using a multiplex PCR-based technique able to amplify the known Qnr variants, as previously described (16 ).
Proximity ligation assay (PLA) is a highly sensitive technique for multiplex detection of biomarkers in plasma with little or no interfering background signal.We examined the plasma levels of 21 biomarkers in a clinically defined cohort of 52 locally advanced (Stage II/III) pancreatic ductal adenocarcinoma cases and 43 age-matched controls using a multiplex proximity ligation assay.
Using a multiplex PCR assay, X. oryzae pv.
The levels of serum proinflammatory and antiinflammatory cytokines were measured using a multiplex bead immunoassay.
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
IL-6 was measured using a multiplex panel (BioRad).
Plasma samples were analysed using a multiplex ELISA.
Multiplex PCR for the polymorphic markers and the mutation was then carried out using a multiplex PCR kit (Qiagen Multiplex PCR kit; Qiagen, Venlo, The Netherlands).
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