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Cytokines were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem, Billerica, MA).
Murine interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), IL-12 p70, interferon-γ (IFN-γ), IL-18, IL-17, IL-23, transforming growth factor-β1 (TGF-β1), mast cell proteinase-1 (MCP-1), and macrophage inflammatory proteins-1 (MIP-1) were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem Billerica, MA, USA).
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Proinflammatory cytokines (IL-1 β, IL-6, INF- γ, and TNF- α) were analyzed using a multiplex sandwich ELISA (SearchLight, Pierce Biotechnology, Rockford, IL, USA) according to the manufacturer's instructions.
This is a multiplex sandwich-ELISA in a planar, plate-based array format, including SignatureLUS CCD Imaging and ProArray Analysis System.
IgG anti-oxLDL antibodies were measured using an in house sandwich-ELISA, of which details have been described earlier [ 10].
The serum MBL level was measured using a sandwich ELISA (MBL-ELISA; Dobeel, Gyeonggi, Korea) according to a previously established protocol [ 23].
Aβ1 40 and Aβ1 42 were measured using a sensitive sandwich ELISA system.
Aβ levels were quantified using a sensitive sandwich ELISA as previously described (Blurton-Jones et al., 2009).
Concentration of ALT-836 in serum samples was determined using a validated sandwich ELISA, as described previously [ 14].
Parieto-temporal cortical Aβ42 levels were measured by using a sensitive sandwich ELISA kit (Invitrogen, Camarillo, CA).
The IL-6 sandwich ELISA was performed using a sandwich ELISA kit (Biosource International, Camarillo, CA, USA) according to the manufacturer's instruction.
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