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The extracted nucleic acid was tested using a multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay.
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The mosquito and swine specimens were subjected to viral RNA detection by using a multiplex reverse transcription PCR (RT-PCR).
The washings were analyzed by using the eSensor Respiratory Panel (GenMarkDx, Carlsbad, CA, USA), a multiplex, reverse transcription nucleic acid amplification test designed to detect influenza viruses A and B, RSV, and rhinovirus.
A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) was developed for CSFV and PRRSV co-infections or infections, respectively.
In this study a multiplex Reverse Transcription-PCR (RT-PCR) was developed and optimized for simultaneous detection and differentiation of the three latent viruses and apscarviroids.
This study was designed to determine the frequency of BCR-ABL transcripts using one-step multiplex reverse transcription polymerase chain reaction (RT-PCR).
Group A rotaviruses were detected in stools collected from diarrhea patients by ELISA and genotyped using multiplex reverse transcription PCR followed by nucleotide sequencing.
A total of 99 of the 285 HRSV-positive NPA samples were randomly selected for subtyping using multiplex reverse transcription polymerase chain reaction; 10 and 79 NPA samples were identified as subgroup A and subgroup B, respectively.
Subsequently, PED was diagnosed in a private laboratory (IVD GmbH, Hannover, Germany) by use of a published multiplex reverse transcription quantitative PCR (7 ).
Norovirus genogroups GI and GII were detected by using a multiplex PCR specific for synthesized complementary DNA after viral RNA extraction and reverse transcription.
Identification of influenza type and influenza A subtypes was conducted using multiplex nested reverse-transcription polymerase chain reaction (RT-PCR) as previously described [ 21, 22].
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