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Concentrations of released cytokines were measured using a multiplex rat cytokine immunoassay (Milliplex map rat cytokine/chemokine panel, Millipore, Billerica, MA).
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In the present study, we investigated the kinetics of 24 cytokines in rats developing AIA using a multiplex immunoassay that allowed a highly sensitive biological follow-up of multiple mediators from a limited amount of biological sample [ 15].
Aeroallergen content (house dust mites (Der p1), cockroach (Bla g2), cat (Fel d1), dog (Can f1), rat (Rat n1) and mouse (Mus m1) was determined with a MARIA kit (Indoor Biotechnologies, Charlottesville, USA) using a multiplex bead based assay.
Using a multiplex PCR assay, X. oryzae pv.
SCCmec type assignment was performed using a multiplex PCR method, as described previously [22].
Cytokines were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem, Billerica, MA).
cDNA was synthesized from total RNA using a Multiplex RT for Taqman array kit (Applied Biosystems, Foster City, CA).
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
IL-6 was measured using a multiplex panel (BioRad).
Plasma samples were analysed using a multiplex ELISA.
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