Protein expression of nine angiogenic cytokines and eight MMPs was analysed using a multiplex-based protein assay.
Kinase signaling was assessed on homogenized kidney lysates using a multiplex, bead-based suspension protein array service (AssayGate Inc, Ijamsville, MD).
We note here that we have changed the title in line with suggestions from the reviewers and eLife editors to be, "Selection of chromosomal DNA libraries using a multiplex CRISPR system". 1) In the "protein evolution" experiment, a single cycle of mutation was done and it is not clear how more complex selection procedures would be implemented.
All the cytokine analysis (leptin, adiponectin, monocyte chemoattractant protein-1 and tumor necrosis factor) were performed using a Multiplex Adipokine/Adipocyte Panels (Assay Gate, Inc, Ijamsville, MD, USA).
Protein expression of inflammatory factors and an angiogenic cytokine was detected using a multiplex cytokine assay in RPE cells treated with A2E or A2E combined with 3-MA.
Using a multiplex PCR assay, X. oryzae pv.
Insulin, adipokines and cytokines were quantified using a Multiplex system (MADKMAG-HK, Millipore).
The levels of serum proinflammatory and antiinflammatory cytokines were measured using a multiplex bead immunoassay.
The protein levels of IL-1β, IL-12p70, IFNγ, IL-6, IL-10, Cxcl1 and TNFα in adipose tissue lysates were measured using a multiplex (7-plex) ELISA (Meso Scale Discovery, Gaithersburg, MD).
