IL-1β and TNF-α in human plasma, mouse plasma, and mouse lung homogenates were detected by immunofluorescence using a multiplex panel assay (R&D Systems, Minneapolis, MN).
IL-6 was measured using a multiplex panel (BioRad).
Additionally, we assessed the cytokine/ chemokine profile in serum samples from mice at the acute and chronic phases of TB infection and during therapy and investigated the diagnostic value of 17 immunological markers using a multiplex panel of bead-labelled cytokine and chemokine receptors.
Another study analyzed 17 ACPAs using a multiplex panel similar to the panels used in our study in stored serum samples from the US Department of Defense Serum Repository, including 81 individuals who had blood samples taken before RA diagnosis [ 6].
As all isolates were stored, capsular serotyping could be performed using a multiplex PCR panel.
Using a multiplex immunoassay panel, Sabine Bahn (University of Cambridge, UK) reported a molecular signature in plasma in first-episode schizophrenia.
A major consideration when deciding between a targeted, single-gene approach to genetic testing versus testing using a multiplex gene panel is the increased chance of receiving inconclusive results, which presents challenges for both patients and clinicians.
All the cytokine analysis (leptin, adiponectin, monocyte chemoattractant protein-1 and tumor necrosis factor) were performed using a Multiplex Adipokine/Adipocyte Panels (Assay Gate, Inc, Ijamsville, MD, USA).
All the cytokine analysis (leptin, adiponectin, monocyte chemoattractant protein-1 and tumor necrosis factor) were performed using a Multiplex Adipokine/Adipocyte Panels (Assay Gate, Inc, Ijamsville, MD, USA) according to the manufacturer's instructions.
Rules-based medicine (RBM; Austin, TX, USA) evaluated CSF samples using a multiplex Human DiscoveryMAP™ panel consisting of 159 analytes (including C3) on a Luminex 100 platform (Luminex Corp, Austin, TX, USA).
Serum obtained from mice was analyzed for adipokines and bone panel measurements using a multiplex mouse adipokines assay (Mouse adipocyte panel, Millipore, Bedford, MA, USA), and detected by Luminex xMAP method (Luminex 200, Millipore, Bedford, MA, USA).
