Additionally, we assessed the cytokine/ chemokine profile in serum samples from mice at the acute and chronic phases of TB infection and during therapy and investigated the diagnostic value of 17 immunological markers using a multiplex panel of bead-labelled cytokine and chemokine receptors.
To ensure testing groups were well balanced and directly comparable, four animals, one animal from each four testing groups (young, 15 days old, and old, 30 days old, of male control (CT) and A30P flies) were evaluated in parallel using a multiplex arena of 6 cm in diameter for imaging and tracking (Fig. 2).
The presence of HPV was assessed using a multiplex PCR system of 10 non-biotinylated primers.
We measured cytokine production by withdrawal of supernatant from infected microglia cultures and quantification using a multiplex assay and found production of IL-8, IL-10, and TNF-α, which increased linearly.
SCCmec typing was performed using a multiplex PCR strategy with sets of region-specific primers as previously described [37].
In addition, a representative S. epidermidis strain collection has been analyzed by using a multiplex PCR and the frequency of certain prophage groups determined.
In preliminary studies using a multiplex phosphoprotein assay, specific ablation of EpCAM decreased c-Jun and JNK1 phosphorylation in breast cancer cells.
Rules-based medicine (RBM; Austin, TX, USA) evaluated CSF samples using a multiplex Human DiscoveryMAP™ panel consisting of 159 analytes (including C3) on a Luminex 100 platform (Luminex Corp, Austin, TX, USA).
Oxidase, string test, and arginine dihydrolase positive strains that did not agglutinate with V. cholerae O1 polyvalent or O139 monovalent antiserum were further confirmed as V. fluvialis by using a multiplex PCR targeting the toxR gene of V. fluvialis and the ompW gene of V. cholerae (8, 9 ).
Portal concentrations of glucose-dependent insulinotropic peptide (GIP), glucagon-like peptide-1 (GLP-1), ghrelin and leptin were determined in 2 × 15 µl of plasma using a multiplex immunoassay kit (Bioplex, Bio-Rad) and measured using Luminex technology (Bioplex, Bio-Rad).
