The total IgG and IgA concentration of mouse vaginal lavage was determined using a multiplex mouse immunoglobulin isotyping kit (Millipore, Billerica, MA) following the manufacturer's instructions.
Serum obtained from mice was analyzed for adipokines and bone panel measurements using a multiplex mouse adipokines assay (Mouse adipocyte panel, Millipore, Bedford, MA, USA), and detected by Luminex xMAP method (Luminex 200, Millipore, Bedford, MA, USA).
Concentrations of IL-4, IL-5, IL-13, IL-17 and IFN-γ in serum were measured using a multiplex Mouse Cytokine Magnetic Bead Panel (Millipex Map Kit; EMD Millipore Corp ,Billerica, MA, USA).
The concentration of proinflammatory cytokines in BAL fluid and in culture supernatants was assessed either using enzyme immunoassay kits (R&D Systems, Sydney, Australia) or using a multiplex immunoassay (Mouse 23-Plex panel, Biorad Laboratories, Hercules, CA, USA), according to the manufacturer's instructions.
Twenty-three acute phase and pro-inflammatory cytokines were quantified in matched serum, EDTA-plasma, and heparin-plasma samples from anesthetized and unanesthetized male NMRI mice using a multiplex assay.
IL-1β and TNF-α in human plasma, mouse plasma, and mouse lung homogenates were detected by immunofluorescence using a multiplex panel assay (R&D Systems, Minneapolis, MN).
A maximum of 16 microsatellite loci was used for paternity analyses (D11Mit150, D9Mit34, D9Mit135, D17Saha, D17Mit28, D10Mit20, D2Mit252, D6Mit138, D15Mit16, D5Mit25, D19Mit39, D7Mit227, D1Mit456, D2Mit380, D17Mit21, D1Mit404, see Mouse Microsatellite Data Base of Japan) using a Multiplex PCR MasterMix (Qiagen Multiplex PCR kit).
Cytokine detection in clarified tissue lysates was performed using a mouse cytokine panel (Millipore, Billerica, MA) with detection using a multiplex platform (Luminex, Austin, TX).
Mouse plasma was also used to measure cytokine levels using a multiplex bead-based assay.
Aeroallergen content (house dust mites (Der p1), cockroach (Bla g2), cat (Fel d1), dog (Can f1), rat (Rat n1) and mouse (Mus m1) was determined with a MARIA kit (Indoor Biotechnologies, Charlottesville, USA) using a multiplex bead based assay.
Using a multiplex PCR assay, X. oryzae pv.
