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Total RNA was extracted and mRNA expression analyzed using a multiplex low-density array qRT-PCR platform, or single-gene qRT-PCR assay.
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Using a multiplex PCR assay, X. oryzae pv.
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
IL-6 was measured using a multiplex panel (BioRad).
Plasma samples were analysed using a multiplex ELISA.
Multiplex PCR for the polymorphic markers and the mutation was then carried out using a multiplex PCR kit (Qiagen Multiplex PCR kit; Qiagen, Venlo, The Netherlands).
Plasma fractions were analyzed using a multiplex-microbead immunoassay.
Estrogen-regulated miRNAs were identified by using a TaqMan low density array.
Validation of the microarray data was achieved using a custom Taqman Low Density Array card (Applied Biosystems).
Using a commercial kit (Parsazmun, Iran), low-density lipoprotein (LDL) was measured.
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