Sentence examples for using a multiplex ligase from inspiring English sources

Infant genotyping of CCR2-64I and CCR5 promoter polymorphisms were determined using a multiplex ligase detection reaction (LDR) based method with flow cytometric technology, previously described [43], [44].

Genetic identification of enterococci to species level and detection of vanA, vanB, vanC1, or vanC2/C3 and vanD ligase genes were performed by using a multiplex polymerase chain reaction amplification (7 ).

Using a multiplex PCR assay, X. oryzae pv.

SCCmec type assignment was performed using a multiplex PCR method, as described previously [22].

Cytokines were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem, Billerica, MA).

cDNA was synthesized from total RNA using a Multiplex RT for Taqman array kit (Applied Biosystems, Foster City, CA).

Genotyping was performed using a multiplex protocol (see Supporting Information).

Molecular serotyping was completed using a multiplex PCR scheme [ 61].

Target DNA sequences were amplified using a multiplex PCR method and ligation reactions for each subject were carried out in a final volume of 10 μL containing 1 μL of 10× buffer, 100 ng of multi-PCR product, 1 pmol of each discriminating oligo, 1 pmol of each common probe, and 2 U of Taq DNA ligase (New England Biolabs, Beverly, MA, USA).

IL-6 was measured using a multiplex panel (BioRad).

Plasma samples were analysed using a multiplex ELISA.