Sentence examples for using a multiplex kit from inspiring English sources

Leptin, resistin, insulin, total plasminogen activator inhibitor-1 (tPAI-1), interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1) were measured using a multiplex kit and adiponectin, a single plex kit (Linco Research, St. Charles, MO, USA) on a Luminex system (Austin, Texas, USA).

Circulating serum cytokines levels were measured using a Multiplex kit from Meso Scale Discovery according to the manufacturer's protocol.

Porcine cytokines (n = 9) were analyzed in plasma using a multiplex kit from Bio-Rad Laccordinges, Inc. according to the manufacturer's instructions.

To increase confidence in the biological interpretation of our microarray data, we determined the concentrations of three cytokines and a chemokine (IL-10, IL-6, IL-1β and MIP-1β), using a multiplex kit.

For instance, Heesen et al. [ 8] used ELISA (enzyme-linked immunosorbent assay) to analyse cytokines, whereas we used a multiplex kit.

Multiplex PCR for the polymorphic markers and the mutation was then carried out using a multiplex PCR kit (Qiagen Multiplex PCR kit; Qiagen, Venlo, The Netherlands).

Microsatellite markers were amplified using polymerase chain reaction (PCR) in a 10 μl reaction using a multiplex PCR kit (Qiagen Inc .. PCR conditions for each reaction using the multiplex kit were 5 μl of Qiagen Master Mix (containing HotStarTaq DNA polymerase, dNTPs and 3 mM MgCl2), 0.625 μM of each primer, and 3 μl of genomic DNA.

PCRs were carried out using a multiplex PCR kit (Qiagen, Germantown, Maryland, US) in 5 μL final volumes and using 5 ng of DNA as template.

PCR was performed using a multiplex primer kit (Invivoscribe Technologies, San Diego, CA) specific for a majority of clonal TCR β chain rearrangements [28].

Lungs were washed with 2.5 ml of 1x PBS to retrieve the BAL, the fluid was centrifuged and cytokines/chemokines were estimated in the BAL fluid using a multiplex flowcytomix kit (Bender MedSystems).

The reactions were carried out in 5 μL final volumes using 10 ng DNA template using a multiplex PCR kit (Qiagen).