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Lungs were washed with 2.5 ml of 1x PBS to retrieve the BAL, the fluid was centrifuged and cytokines/chemokines were estimated in the BAL fluid using a multiplex flowcytomix kit (Bender MedSystems).
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Using a multiplex PCR assay, X. oryzae pv.
Cytokines were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem, Billerica, MA).
cDNA was synthesized from total RNA using a Multiplex RT for Taqman array kit (Applied Biosystems, Foster City, CA).
SCCmec type assignment was performed using a multiplex PCR method, as described previously [22].
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
IL-6 was measured using a multiplex panel (BioRad).
Plasma samples were analysed using a multiplex ELISA.
Multiplex PCR for the polymorphic markers and the mutation was then carried out using a multiplex PCR kit (Qiagen Multiplex PCR kit; Qiagen, Venlo, The Netherlands).
Mouse plasma was also used to measure cytokine levels using a multiplex bead-based assay.
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