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To gain a broader understanding of vaccine-induced T-cell responses, we tested these samples for thirty secreted cytokines in response to Gag peptide stimulation using a multiplex cytokine assay that we developed.
Supernatants were cleared by centrifugation and analyzed using a multiplex cytokine assay.
In order to address this, we screened for multiple cytokines, using a Multiplex cytokine assay system (MCYTOMAG-70 K-PMX).
Protein expression of inflammatory factors and an angiogenic cytokine was detected using a multiplex cytokine assay in RPE cells treated with A2E or A2E combined with 3-MA.
T-cell line supernatants were then assayed for cytokines using a multiplex cytokine bead array system (cat. no. 171-A11050 Bio-Plex; Bio-Rad, HerCAles, CA, USA) according to the manufacturer's instructions.
Serum samples were assayed in duplicate for concentrations of leptin, IL-1β, IL-6, MCP-1, and TNF-α using a multiplex cytokine assay (Millipore Milliplex, cat no. RCYTOMAG-80 K; Billerica, MA, USA) on a MAGPIX instrument (Luminex Technologies; Luminex, Austin, TX, USA) according to the manufacturer's instructions.
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In order to determine whether the changes in the mRNA level results in a parallel increase in the secretion of the cytokines and chemokines in the cellular supernatants, we used a multiplex cytokine assay to examine the secretion of cytokines and chemokines in the supernatants of vector- and K13-ERTAM-expressing HUVECs that had been either mock treated or treated with 4OHT for 48 hours.
Concentrations of released cytokines were measured using a multiplex rat cytokine immunoassay (Milliplex map rat cytokine/chemokine panel, Millipore, Billerica, MA).
Concentrations of IL-4, IL-5, IL-13, IL-17 and IFN-γ in serum were measured using a multiplex Mouse Cytokine Magnetic Bead Panel (Millipex Map Kit; EMD Millipore Corp ,Billerica, MA, USA).
Additionally, we assessed the cytokine/ chemokine profile in serum samples from mice at the acute and chronic phases of TB infection and during therapy and investigated the diagnostic value of 17 immunological markers using a multiplex panel of bead-labelled cytokine and chemokine receptors.
To investigate the relation between functional impairments of cancer patients and circulating cytokines using a multiplex technique.
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