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Angiogenin levels were determined by ELISA (RayBiotech, Norcross GA, USA) whereas the concentration of the other cytokines was done using a multiplex assay (Bioplex, Bio-Rad, Switzerland) following the manufacturer's instructions.
The inflammatory cytokines IL-8, IL-1β, MIP-1β and MCP-1 were measured in undiluted monocyte supernatants using a multiplex assay on the BioPlex 2200 platform (Bio-Rad, Hercules, CA, USA), with commercial antibody-coated beads, standards, and reagents, according to the manufacturer's instructions (Bioplex human cytokine multiplex assay system, BioRad).
MLVA profiles were generated using a multiplex assay targeting five VNTR loci [ 69].
In addition, the identification of several GM targets using a multiplex assay is not applicable [ 28].
Secreted cytokines and chemokines in cell culture supernatants were analysed using a Multiplex assay.
Adiponectin levels were measured by using a multiplex assay kit (EMD Millipore, Billerica, MA, USA) in accordance with the instructions of the manufacturer.
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We used a multiplex assay to measure levels of 25 cytokines and chemokines in plasma samples from 20 AIDS patients in the study cohort and explore their relationship to individual IBD serological markers.
Samples were stored at −20°C until analysis using a multiplex bead assay (Millipore Milliplex assay), conducted according to the manufacturer's instructions and read using a Luminex 100 plate reader.
All blood and CSF samples were assayed using a multiplex PCR assay for the detection of N. meningitidis, H. influenzae and S. pneumoniae as previously described [18].
All isolates were subjected to screening using a multiplex PCR assay for the detection of qnr types (A, B, C, and S) [ 13] and a monoplex PCR assay for the detection of qnrD type [ 30].
Using a multiplex PCR assay, X. oryzae pv.
More suggestions(15)
using a multiplex rat
using a multiplex primer
using a multiplex mouse
using a multiplex sandwich-ELISA
using a multiplex low-density
using a multiplex panel
using a multiplex cytokine
using a multiplex array
using a multiplex reverse-transcription
using a multiplex bead
using a multiplex ligase
using a multiplex immunoglobulin
using a multiplex flowcytomix
using a multiplex kit
using a multiplex protein
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