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Cytokine levels were determined using a multiplex array reader from Luminex™ Instrumentation System (Bio-Plex Workstation from Bio-Rad Laboratories). Bio-Rad Laboratories
Cytokine levels were determined using a multiplex array reader from Luminex™ Instrumentation System (Bio-Plex Workstation from Bio-Rad Laboratories, USA).
Endotoxin levels were assayed by a Limulus amebocyte lysate assay (Lonza, Karlsruhe, Germany), and allergens were measured using a multiplex array for indoor allergens (MARIA; Indoor Biotechnologies, Charlottesville, VA), according to the manufacturer's instructions.
Nine cytokines (TNF- α, IL-1 β, IL-6, IL-12p40, IL-18, IFN- α2, IL-4, IL-8, and IL-10) were evaluated using a multiplex array, and we were able to detect four of these cytokines in cell culture supernatants (TNF- α, IL-6, IL-8, and IL-10), but the level of expression of the remaining cytokines was below the detection limit.
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We used a multiplex array to measure reactivities against the arginine and citrulline variants of 11 different antigens.
We used a multiplex array format with an average probe length of 36 nucleotides and were able to detect significantly differentially expressed genes.
Serum from 234 subjects enrolled in a CT screening cohort was analyzed for 33 cytokines and growth factors using a multiplex protein array.
In addition, both systemic and local cytokine levels were determined in sera and patellae washouts, using a multiplex bead array (Luminex Technology).
Lee et al. [ 61] analyzed saliva samples using a multiplex protein array for selected biomarkers implicated in host defense, inflammation, tissue destruction and angiogenesis, and measured subgingival bacterial proportions by checkerboard DNA-DNA hybridization.
The patients were checked for 17 kinds of cytokines (IL-1, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNFα, G-CSF, GM-CSF, IFNγ, MIP-1, MCP-1/MCAF) using a multiplex suspension array system.
Total RNA was extracted and mRNA expression analyzed using a multiplex low-density array qRT-PCR platform, or single-gene qRT-PCR assay.
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