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Using a multiplex analysis, this showed a detectable level of α globin in 12% of OCT4 positive ES cells.
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Samples were stored at −20°C until analysis using a multiplex bead assay (Millipore Milliplex assay), conducted according to the manufacturer's instructions and read using a Luminex 100 plate reader.
Mice were bled at 3 hours for serum cytokine analysis using a multiplex bead assay, per the manufacturer's protocol using their software and hardware (Bio-Plex, Bio-Rad).
Using a multiplex bead based system, allowing simultaneous analysis of over 20 soluble molecules in very small sample volumes [ 13], we measured a panel of cytokines and chemokines in the synovial fluid of patients with early inflammatory arthritis.
Analysis will be performed using a multiplex ELISA based platform Serum will be separated and frozen at −80°C until analysis.
All the cytokine analysis (leptin, adiponectin, monocyte chemoattractant protein-1 and tumor necrosis factor) were performed using a Multiplex Adipokine/Adipocyte Panels (Assay Gate, Inc, Ijamsville, MD, USA).
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
IL-6 was measured using a multiplex panel (BioRad).
Plasma samples were analysed using a multiplex ELISA.
Multiplex PCR for the polymorphic markers and the mutation was then carried out using a multiplex PCR kit (Qiagen Multiplex PCR kit; Qiagen, Venlo, The Netherlands).
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