We then describe an in vivo gain-of-function genetic experiment using a mouse line carrying a functional Map3k4 bacterial artificial chromosome (BAC) transgene.
Gnerre et al. [ 12] briefly explored the effect of using different read coverage levels in a mouse chromosome assembly.
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Below, we demonstrate this protocol for ChIP enrichment using mouse chromosome 16 tiling microarrays (Agilent Technologies), followed by subsequent deep sequencing using an Illumina/Solexa 1G Genome Analyzer.
The generation of transgenic mice, using a bacterial artificial chromosome construct to drive Venus expression by the proglucagon promoter is described in detail elsewhere (7).
By inserting an extra chromosome into mouse embryos (they used mouse Chromosome 16, a portion of which is analogous to human Chromosome 21), they produced a mouse that exhibited many of the characteristics of Down syndrome.
Expression of human FMRP, using a yeast-artificial chromosome transgenic mouse model, corrected several altered behaviors, including normalized locomotor activity, normalized anxiety-like responses to novel food, and normalized social approach [18].
PL10 was first identified in mouse by using a human Y chromosome (Chr) derived probe [ 1] and is present in a wide range of eukaryotes from yeast, plants, and animals, including humans [ 2].
In the present study, we have generated a transgenic mouse line using a bacterial artificial chromosome (BAC) clone containing 207 kb of rat BDNF (rBDNF) locus, encompassing the genomic region from 13 kb upstream of rBDNF exon I to 144 kb downstream of rBDNF coding exon.
Using a bacterial artificial chromosome (BAC) transgenic mouse line, in which the enhanced green fluorescence protein (EGFP) reporter gene expression was driven by the PK2 promoter, we were able to obtain an efferent projections map from the EGFP-expressing neurons in the SCN.
