The discs were homogenized in 20 µl cell lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% Nonidet P-40) containing protease inhibitors using a motor driven small plastic pestle.
Glass microelectrodes with a tip diameter of less than 100 µm (Unisense, Aarhus, Denmark; Diamond General, Ann Arbor, MI, USA) were deployed using a motor driven micromanipulator (National Aperture, NH, USA) in combination with a picoammeter (Unisense) and high-impedance millivolt meter (Microscale Measurements, The Hague, The Netherlands), for O2 and Ca2+, respectively.
The tissues were then homogenized at 4°C in five volumes of homogenizing buffer per gram of tissue using a motor driven glass-Teflon Potter-Elvejhem homogenizer.
20% tissue homogenates of muscle, gill, and hepatopancreas were prepared in 25 mM PB containing 2 mM EDTA and Triton X-100 (0.1%, v/v), pH 7.6, using a motor driven glass-Teflon homogenizer at 4°C.
The ovary, hemolymph, and hepatopancreas samples were collected from control and experimental prawns, shock frozen in liquid nitrogen, and homogenized individually on ice in 100 μL deionized water using a motor driven Teflon pestle.
The pellet containing the nuclei was resuspended in ice cold buffer (50 mM Tris-HCl, pH 7.4 at 22°C, 1.5 mM sodium EDTA, 10% glycerol, 10 mM sodium molybdate and 10 mM monothioglycerol) containing 0.4 M KCl and homogenized on ice with a Dual glass-glass homogenizer using a motor driven pestle.
Nissan's Variable Valve Event and Lift technology operates similarly to BMW's valve-throttling system, using a motor-driven shaft and levers to vary intake valve lift in response to driver throttle input and other factors.
The knee angle of extension was measured using a motor-driven arthrometer.
The children seen in Rotterdam performed a graded, maximum exercise test using a motor-driven treadmill.
Frozen tissue was homogenised in Trizol™ (Invitrogen) using a motor-driven homogenizer (Polytron, Kinematica, Newark, NY, USA).
Ports were drilled into the glass microscope slide, and flow was controlled using a motor-driven syringe pump (Amitani et al., 2010; Forget and Kowalczykowski, 2010, 2012).
