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However, it might be possible to apply our findings to general medical students by using a more strict definition of peer social support.
All included systems incorporated some form a hierarchical approach with three systems (Wigglesworth, Aberdeen and ReCoDe) using a more strict approach than the others.
In future studies, we suggest using a more strict definition of CAM therapies and asking exactly the same questions in conditions to be compared.
To exclude diabetes using a more strict definition in the FOS cohort, we analyzed data from nondiabetic subjects who had both FPG <126 mg/dl and 2-h postload glucose <200 mg/dl.
Among studies that included all self-reported cases (11, 13, 26, 27, 33), 2 stated that validity of self-reports was reasonable (13, 33) and 1 that results did not differ substantially when using a more strict case definition (11).
Of note, when we have rescored our data using a more strict definition of hypopneas based only on a minimum of 4% desaturation (without considering the arousals), only 2 of the 52 subjects (3.8%) were categorized as having OSA (AHI >5), which suggests that using only oxygen criteria, the presence of OSA may not be captured in young lean populations.
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We also used a more strict criterion for ESR change between two species taking into account overlapping hexamers [40]: for any basepair change that introduces a ESE or ESS in one species, none of the corresponding 6 overlapping hexamers in the other species can be an ESE or ESS, respectively.
Rasmussen et al. used a more strict PFFn of 25% whereas we used a PFFn of 15%.
For determination of bivalent promoters, we wished to use a more strict criterion because we wanted a set that had strong signals for both epigenetic modifications (either H3K4me3+/H3K27me3+ or H3K4me3+/H3K9me3+).
However, we applied a targeted approach, and if we had used a more strict selection of putative SNPs this would, in our case, decreased the total number of putative SNPs rather than led to selection of other putative SNPs in the target genes that were more likely to pass the genotype validation.
Compared to prevalence values reported in previous studies, those found in our study for pure cortical, nuclear, or PSCO types were lower, whereas more cataracts were classified as the mixed type, possibly because we used a more strict classification system for evaluating the risk factors associated with the pure subtype.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com