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Figure 10 The excitation spectrum of the Eu3+ions-doped mesoporous silica thin film with an Eu/Si molar ratio of 3.41% calcined at 600°C for 4 h measured using a monitor wavelength of 615 nm.
The excitation spectrum of the Eu3+ions-doped mesoporous silica thin film with an Eu/Si molar ratio of 3.41% calcined at 600°C for 4 h was measured using a monitor wavelength of 615 nm and is shown in Fig. 10.
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Peptide elution was monitored using a wavelength of 214 nm and with Chromeleon software, version 6.50 (Dionex).
EGFP expression was monitored using a 388-nm wavelength (blue) LED light source, the appropriate cut-off filters and a conventional camera.
Ca 2+ microfluorimetry and imaging Cytoplasmic free calcium concentration ([Ca2+]i) in intact cells loaded with fura-2AM (1 μmol/l; Invitrogen) was monitored using a dual-wavelength Photon Technology International (Birmingham, NJ, USA) system [ 22].
The running buffer was 100 mM sodium phosphate at pH 7.2 with 100 mM NaCl, and the column eluate was monitored using a variable-wavelength UV detector at 220 nm.
The fluorescence was monitored using an excitation wavelength of 650 nm and an emission wavelength of 680 nm.
Tryptophan fluorescence was monitored using an excitation wavelength of 294 nm and emission wavelength of 350 nm.
Tyrosine fluorescence signal was monitored using an excitation wavelength of 280 nm and emission wavelength of 305 nm.
ThT fluorescence was monitored using an excitation wavelength of 450 nm and emission wavelength of 480 nm.
Fluorescence measurements were carried out on a Varian Cary Eclipse fluorimeter (Varian Ltd., Oxford, UK) using a 1 cm path length quartz cuvette (Starna, Essex, UK), and dityrosine fluorescence was monitored using an excitation wavelength of 320 nm.
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