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CAT activity was measured by following the rate of H2O2 decomposition, which can be determined directly by the decrease in absorbance at 240 nm, using a molar extinction coefficient of 22.2 × 103 M−103m−103
Reaction rates were calculated using a molar extinction coefficient of 36 mM/cm.
Content of TBARS was expressed in micromoles of malondialdehyde using a molar extinction coefficient 1.55 × 105 cm−105−105
Concentration was determined spectrophotometrically using a molar extinction coefficient of TAMRA (80,400 M−1 cm−1) at 547 nm.
AChE activity was calculated using a molar extinction coefficient 1.36 × 10 − 4 M − 1 cm − 1 and expressed in μmol− 1 min − 1 mg protein− 1.
GSH content was calculated using a molar extinction coefficient of 13.6 × 103 M−1 cm−1 and reported as nM/mg protein (Moron et al. 1979).
The concentration of malondialdehyde (MDA) was monitored at 535 and 600 nm using a Perkin Elmer Lambda 40 spectrophotometer, and the MDA concentration was calculated using a molar extinction coefficient of 155 mM cm−1.
The reduction of NADPH to NADP+ was recorded at 340 nm, and the concentration of NADPH was calculated using a molar extinction coefficient of 6.22 × 103 M-1 cm-1.
Pigment content was calculated as milligrams cyanidin-3-glucoside per 100 g fresh fruit or jam using a molar extinction coefficient of 26 900 L/cm/mol and molecular weight of cyanidin-3-glucoside (449.2 g/mol).
The monomeric anthocyanins content was expressed as mg per 100 g fresh bilberries, respectively jam using a molar extinction coefficient of 26 900 and a molecular weight of 449.2 of the most prevalent anthocyanin (usually cyanidin-3-glucoside).
Other hemoproteins' concentrations were determined by calculation using a molar extinction coefficient of ∼105 for the Soret band.
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