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The yield was elevated to 31 % by using a molar equivalent of TMEDA and 2.1 equiv. of ArMgBr (Table 1, entry 11).
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Calculations were made using a molar extinction coefficient of 9.6 mM−1 cm−1.
Optical density was measured at 340 nm, using a molar coefficient of 9.61 × 10/M/cm.
Units were calculated using a molar absorptivity of 2.66 × 104 M−1 cm−1 for tetraguaiacol or 3,3′-dimethoxy-4,4′-biphenoquinone 3,3′-dimethoxy-4,4′-biphenoquinone 3,3′-dimethoxy-4,4′-biphenoquinone 3,3′-dimethoxy-4,4′-biphenoquinone
Hydrogen peroxide was calculated as nmol H2O2/g tissue using a molar extinction coefficient of 43600 M−1 cm−1.
Enzymatic activity was calculated using a molar extinction coefficient of 6700.
Carbonyl content was determined at 370 nm using a molar absorption coefficient of 22,000 M−1 cm−1 [ 18].
The carbonyl content was calculated using a molar absorption coefficient of 22,000/M-cm = ɛ.
The protein concentration was estimated using a molar extinction coefficient at 280 nm of 9080 M−1 cM−1.
However, when fluorescein was dissolved in DCM, solvated using TEA, followed by addition of AcOH (a molar equivalent), on addition of an aqueous solution of SDS, the fluorescein remained in the organic phase (Figure 3).
Biotinylated Cd4d3 + 4-tagged recombinant proteins were immobilized onto streptavidin-coated SA sensor chip surfaces at low levels (Flrt3 ~750 RU, Jamc.2 ~400 RU, Lrrn1 ~1320 RU) and a molar equivalent of Cd4d3 + 4bio used as a reference.
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