The targeting vector was also generated by recombineering using a modified pL253 vector containing homologous segments of RP23-451C5 flanking the region of interest.
DBL and CIDR structural information [30], [31], [32] was used to guide the design of domain boundaries, and proteins were expressed using a modified pET15b vector in Origami B cells, supplemented with the pRIG vector [33] to enrich for rare tRNAs [29], [34].
Using a modified piggyBac vector for insertional mutagenesis in a genetic screening 41 and by generation of flies expressing a modified version of cohesin subunit RAD21 42 (named RAD21TEV), they found that cohesin is essential for axon pruning.
Human Brk1 was purified from E.coli using a modified pACYC vector encoding a fusion protein displaying 6xHis, GST, a TEV protease cleavage site then hBrk1.
The CAG-GFP-sponge-bGH-polyA plasmids were self-constructed using a modified pCRII vector as backbone (Life Technologies, Darmstadt, Germany).
In this work we have showed that E2 peptides can be expressed in planta by using a modified PVX vector.
An N-terminally HA-tagged Sam50 candidate (T.v.Sam50) was transformed into T. vaginalis strain C1 using a modified pTagVag vector.
The clone of human Ub, a generous gift of Paul Driscol (National Institute for Medical Research, London, UK), was overexpressed in E. coli strain BL21 as an untagged construct using a modified pET vector.
We expressed the human CXCR4 receptor using a modified baculovirus expression vector that facilitates a single step antibody affinity purification of CXCR4 to >80% purity from Hi5 cells.
YFP tagged EB1 was used to replace one endogenous allele and was stably expressed using a modified pCR4Blunt-TOPO vector [ 22].
