The bismuth nanowires were first synthesized and purified using a modified template-based vapor deposition technique, resulting in nanowire diameters of either 20 or 250 nm, depending on the type of template employed.
In this work, we report on the fabrication of graphitized HCSs and yolk shell Au@HCS nanostructures using a modified templating method, in which smooth, uniform graphene layers were grown on SiO2 spheres or Au@SiO2 nanoparticles via metal-catalyst-free chemical vapor deposition (CVD) of methane.
A fraction of 1 2 μg of total RNA was used for cDNA synthesis with the MINT cDNA synthesis kit (Evrogen, Moscow, Russia), a strategy based on the SMART double-stranded cDNA synthesis methodology using a modified template-switching approach that allows the introduction of known adapter sequences to both ends of the first-strand cDNA.
The normalization process was performed by Evrogen (Moscow, Russia) and based on the SMART double-stranded cDNA synthesis methodology using a modified template-switching approach that allows the introduction of known adapter sequences to both ends of the first-strand cDNA.
When CARAs were reported, a nurse researcher carried out a structured interview with the GP, using a modified Utstein template including location, known patient of the practice, demographic data, times, interventions, rhythms identified, participating services, return of spontaneous circulation (ROSC) on scene or later, discharge from hospital and source of AEDs used.
About 500 ng of gel purified linear mtDNA sample was used for library preparation using a modified 10 kb Template Preparations Protocol (PacBio, University of Washington, Seattle).
RNA-seq libraries were prepared as previously described (Kivioja et al. 2011) with the following changes: 1.8 μg of each RNA sample was used for cDNA synthesis with a modified template switch oligo (5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT- 5 base barcode)-rGrGrG-3′; Eurofins MWG Operon) and libraries were amplified using Advantage 2 PCR system (Clonetech) with 16 cycles of a two-step program.
Initial candidate face locations are identified using a modified version of the ratio template algorithm [Proceedings of the Fifteenth National Conference on Artificial Intelligence (1998)].
Briefly, double-stranded cDNA was prepared from the RNA template using a modified oligo-dT primer containing a 5' T7 RNA polymerase promoter sequence and the Superscript Choice System for cDNA Synthesis (Invitrogen).
To address the role of Hfq and SmpB in Salmonella Typhimurium pathogenesis and protein synthesis we first constructed non-polar deletion mutations of the hfq and smpB genes using a modified pKD13 derivative as the template.
cDNAs were synthesized using the adaptor-ligated mRNAs as template using a modified small RNA RT primer from Illumina (Table 1) and Superscript II Reverse Transcriptase (Invitrogen).
