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The cell lysate is then assayed using a modified protocol for the LANCE cAMP detection kit.
Images were pre-processed and analyzed using a modified protocol for DTI in SPM2.
300 mg of milled seeds were then digested using a modified protocol of dry ashing digestion (Jiang et al. 2007).
For cytokine detection restimulation was performed using a modified protocol.
Gels were blotted and treated with antibodies using a modified protocol [37].
Nuclei were isolated using the Sigma Nuclei EZ Prep Nuclei Isolation Kit using a modified protocol.
LCM was performed using a modified protocol for human pancreatic tissue dehydration [18], [19].
The expressed inclusion bodies were isolated, purified, and refolded using a modified protocol [12], [20], [56], [57].
Cell extracts were prepared using a modified protocol from Rigaut et al. [30] and the NCRR Yeast Resource Center.
Total RNA was extracted from each population of laser captured beta cells using a modified protocol for RNA microisolation [76].
SDS-PAGE was performed using a modified protocol of Laemmli [47] made according to instructions for NuPAGE gels (4 12% Bis-Tris, Invitrogen) and PVDF membranes (Millipore).
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