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The samples were then mixed with formamide gel loading dye and denatured for 10 min at 95°C and then separated on a flashPAGE™ gel apparatus using a modified procedure.
The HNTs and DB-HNTs were prepared using a modified procedure.
Oil extraction for oil content measurements was carried out using a modified procedure according to the AOCS official method [ 79], using a soxhlet apparatus and n-hexane.
Pre- and post PMCA samples were analysed by EIA (IDEXX HerdChek BSE/scrapie EIA antigen test assay) using a modified procedure.
Mycobacterial RNA was recovered from infected host cells 4 and 24 hrs post infection using a differential lysis procedure and amplified using a modified procedure similar to that described by van Gelder et al. ([ 14]; see Methods).
This was done using a modified procedure of the method described by Longin [20].
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Total lipids were extracted using a modified Folch procedure [47] as described by Kraft et al., 2003 [48].
The preparation of the essential oils was done using a modified standard procedure according to the European Pharmacopoeia [39].
4-Phenyl-6-hydroxy-3-cyano-2-pyridone was prepared from ethyl benzoylacetate and cyanoacetamide using a modified literature procedure [14].
Extracts were quantified using a modified Bradford procedure Bio-Rad Laboratoriess, Hercules, CA).
If post-hoc comparisons exceeded the degrees of freedom, p-values of post-hoc single comparisons were corrected using a modified Bonferroni procedure [44].
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