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Extraction of DNA from the samples was conducted using a modified phenol-chloroform procedure [55].
Genomic DNA was extracted using a modified phenol-chloroform protocol.
Genomic DNA was extracted using a modified phenol-chloroform procedure as described by Zhang and Hewitt [ 48].
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Total genomic DNA from ethanol preserved snails was extracted by using their foot tissue and a modified phenol-chloroform extraction protocol followed by purification with GeneClean II kit.
DNA was extracted by proteinase K digestion and a modified phenol-chloroform protocol.
We extracted genomic DNA from peripheral blood samples by proteinase K digestion and a modified phenol-chloroform protocol.
Total RNA was extracted from freeze dried cotyledons and seed coats using a modified McCarty method [ 49] using phenol-chloroform extraction and lithium chloride precipitation.
Total RNA was extracted separately for seven different developmental stages from freeze dried cotyledons using a modified McCarty method [ 79] using phenol-chloroform extraction and lithium chloride precipitation.
Because the southern region contains no true inner- and mid-shelf zones, the genetic structure in this region was explored using pairwise genetic distances Genomic DNA was extracted from approx 0.25 cm2 of fin tissue (re-hydrated by several TE washes) using a modified CTAB extraction procedure [[ 86], excluding the phenol-chloroform step] and re-suspended in 50 μl of TE.
Plasma samples were obtained and RNA and DNA recovered from leukocytes using a modified one-step acid guanidinium thiocyanate-phenol-chloroform extraction (RNA-STAT60, Tel-Test, TX).
RNA and DNA were recovered from leukocytes using a modified one-step acid guanidinium isothiocyanate-phenol-chloroform extraction (RNA-STAT60, Tel-Test, TX).
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