DNA was extracted from each sample directly using a modified bead beating and solvent extraction protocol [12].
The microsatellite markers were developed using a modified magnetic bead protocol (Glenn and Schable, 2005).
Positive clones were selectively retrieved from the plasmid library using a modified version of the magnetic bead cDNA capture method described by Shepard and Rae [ 55].
DNA from the beads was extracted using a modified CTAB extraction method as previously described [22].
Therefore, axial stretch was applied to spontaneously beating SAN cells, using a modified version of the carbon fibre technique.
Using a modified culture technique from Verdugo et al. [ 24] we were able to obtain cultures showing spontaneous ciliary beat with similar characteristics to those of the respiratory epithelium in vivo.
RNA was then isolated from the antibody/bead complex using a modified Ambion RNAqueous kit protocol (Life Technologies, Carlsbad, CA, USA).
Sillett uses a modified arborist-style climbing technique.
Use a modified "shooter" technique.
Use a modified 4-seam grip.
