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DNA was extracted from each sample directly using a modified bead beating and solvent extraction protocol [12].
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Genomic DNA was isolated from M. faveolata sperm using a modified bead-beat protocol.
For B. cereus ATCC 14579 and S. lividans TK24, cells were initially disrupted using a modified bead-beating procedure: cells were resuspended in 400 µl Soil Pro Lysis Buffer (MP Bio), transferred to Lysis Matrix E tubes (MP Bio), and agitated horizontally on a Vortex Mixer (Fisher) with Vortex Adapter (Ambion) for 10 min at speed 10.
The microsatellite markers were developed using a modified magnetic bead protocol (Glenn and Schable, 2005).
Positive clones were selectively retrieved from the plasmid library using a modified version of the magnetic bead cDNA capture method described by Shepard and Rae [ 55].
The correlation between SNP-specific microarray and mass ARRAY-based techniques is shown with high R2 value (0.84) at p-value < 0.001 in Additional file 6. Cotton fibers were mechanically separated from frozen ovular tissue using a modified liquid nitrogen and glass bead shearing procedure [ 47].
The aqueous phase was removed and further processed for RNA isolation, using a modified version of Ambion's bead-based MagMAX-96 Total RNA Isolation kit, done on the MagMAX Express instrument.
RNA was isolated by means of a magnetic bead based approach using a modified version of the Agencourt RNAdvance Blood kit (Beckman Coulter Genomics) and the Hamilton STAR automated liquid handler (Hamilton).
DNA from the beads was extracted using a modified CTAB extraction method as previously described [22].
To generate LSEC proteome, we isolated LSECs from normal and arsenic exposed mice respectively using a modified protocol including two-step collagenase perfusion, centrifugation and magnetic beads sorting, as described previously14,15,16.
The in vivo anti-inflammatory efficacy was evaluated using a modified protocol of carrageenan-induced edema in rat paw administering beads to rats by oral gavage at 0, 3, or 5 h before edema induction.
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