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Using a model substrate to mimic the conglobatin monomer, we also show that the conglobatin cyclase/thioesterase acts iteratively, ligating two monomers head-to-tail then re-binding the dimer product and cyclizing it.
No age-related changes in the activity of AKR1C, assessed using a model substrate 1-acenaphthenol, were observed).
In contrast, no changes in the AKR1C activity, assessed using a model substrate 1-acenaphthenol, were found in rat liver during aging.
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Initial measurements were made to determine the thickness evolution of the poly DMAEMA) nanolayers, using a model flat substrate designed to mimic the three-dimensional nature of initiator incorporation into the membrane.
42, 43 Thus, the four OsII arene iminopyridine complexes were also investigated as transfer-hydrogenation catalysts 42, 43 using a model imine substrate.
DTT-denatured bovine αLactalbumin (Sigma L6010) was used as a model substrate and light scattering at 360 nm was used to monitor protein aggregation in the presence and absence of WT- and mutant HSPB5 at 42°C.
Sucrose-raffinose mixture was used as a model substrate, simulating vinasse, and γ-alumina is also used as an immobilization support for the mixed anaerobic culture leading to increased cell densities in the bioreactor facilitating the repeated batch and continuous processing.
Cyclophosphamide (CYCL), a well-known hepato-activated cytostatic drug was used as a model substrate because of its widespread clinical use.
For endoglucanases, carboxymethyl cellulose (CMC) was widely used as a model substrate.
Interestingly, the NSBs exhibited peroxidase-like activity towards guaiacol used as a model substrate depending on Fenton-like reaction.
To develop appropriate experimental conditions under which the initial reaction velocity of the coupled three-step enzyme reaction directly corresponds to the initial substrate concentration, 1-chlorobutane was used as a model substrate at different concentrations.
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