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Samples were initially ground using a mixer mill.
The tellurium grains were dry milled in air at a frequency of 25 Hz, for 7 h using a Mixer Mill apparatus (Retsch, MM-200).
Figure 5 Comparison of cumulative iron concentration as a function of time for 63 75 μm splits of pyrite HY-001 produced by different grinding methods: hand grinding using an agate mortar and pestle vs. machine grinding using a mixer mill.
Liver was homogenized (1 10 w/v) using a Mixer Mill MM 301 homogenizer (Retsch, Haan, Germany) in ice-cold buffer (0.1 M Tris-HCl, 5 mM ethylenediaminetetraacetic acid (EDTA), pH 7.4), containing a few crystals of phenylmethylsulfonyl fluoride as protease inhibitor.
Alternatively, on a small scale cells were disrupted using a mixer mill (type MM2, Retsch, Haare, Germany).
Lung, skin or heart were disrupted using a mixer mill (Retsch MM301) calibrated for two sessions of 2 min shaking each, at 30 Hz.
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Powder preparation techniques using both a mixer mill and a mortar and pestle were evaluated.
Be careful when using a mixer.
Dried lichens were powdered using a Retsch mixer mill MM 200 (Newtown, PA) in polytetrafluoroethylene grinding jars.
The stems were ground to a homogenised powder using a ball mixer mill (Glen Creston Ltd, Midddlesex, UK), and used to prepare alcohol insoluble residue (AIR).
Leaf tissue was then ground using a Retsch mixer mill (Retsch) in a 2 ml microcentrifuge tube with a tungsten carbide bead for 60 sec until finely powdered.
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