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Further, we found, using a mixed lymphocyte reaction, that the extract was able to significantly reduce CD8+ T cell activity.
We show that this immune tolerizing effect can be co-opted for therapeutic benefit in a mouse model of multiple sclerosis and an assay of organ rejection using a mixed lymphocyte reaction with primary human immune cells.
The ability of BMSCs to suppress alloreactive T-cells has traditionally been assessed in vitro using a mixed lymphocyte reaction (MLR) assay [35].
Tumor-reactive T cells were cultured using a mixed lymphocyte tumor cell culture (MLTC) as described [ 21].
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Using a mixed lymphocyte-endothelial cell culture model we observed that FasL-transfected ECV304 cells which conserved their two principal costimulatory pathways inhibited alloreactive T cell-proliferation by inducing activated T-cell apoptosis.
Triangulation process using a mixed methods approach.
Initially, the histocompatibility of 2-MHC-haplotype-matched MCM was assessed in vitro using a modified mixed lymphocyte reaction (MLR).
Therefore, we next tested the functional ability of these mature DCs to engage allogeneic T cells by using a modified mixed lymphocyte reaction, in which DCs pre-exposed to supernatant were admixed with allogeneic CFSE-loaded T cells (Lyons, 2000).
After reviewing these graphs, exploratory modeling of the relationship between dose/route and select lymphocyte phenotypes was analyzed using a mixed-effects model, with baseline value and actual treatment as fixed effects, and subject as a random effect.
Study protocol using a mixed-methods approach.
Functional assessment of LF-treated DC was performed using these cells as allogeneic stimulators in a mixed lymphocyte reaction (MLR).
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