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We estimated the ages of Elaenia speciation events using a mitochondrial clock rate of 2% sequence divergence/MY for reasons provided in the Discussion.
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Based on these considerations, we have used a mitochondrial clock rate of 2%/MY to date speciation events in Elaenia.
On the other hand, Monteiro and Pierce (2001), using a mitochondrial gene molecular clock estimated the origin of the satyrine genus Bicyclus between 15 and 20 Mya, and the split of the subtribe to which it belongs (Mycalesina: Satyrini) from the subtribe to which Coenonympha belongs (Coenonymphina: Satyrini) at roughly 35 Mya (extrapolated from Figure 1, [ 37]).
We took this as evidence for the presence of molecular clock, or more precisely, if a mitochondrial clock exists in Phasianidae, so does a nuclear clock.
Time judgements were made using a clock hand rotating around a clock face.
By using a special clock calibration method the chip operates from 1.2 to 5 MHz clock frequency.
This prediction will be tested using a molecular clock approach.
Within each graph, two A-scans are displayed, which were calibrated and retrieved through different optical frequency clock signal: the dash line represents using a regular MZI clock; Solid line represents using a log-amplified MZI clock.
As an alternative to the fossil calibration, we used an uncorrelated lognormal (UCLN) relaxed clock (ucld.stdev values for the nuclear genes: 0.635, for the mitochondrial genes: 0.213) and calibrated it using a mean mitochondrial substitution rate of 0.0112 substitutions/site/year x 10−6, which has been calculated for hummingbirds using geographic and habitat-age calibrations [ 39].
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