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Finally, slides were washed four times in PBS and mounted using a minimal volume of faramount mounting medium (DAKO, Carpinteria, CA, USA).
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EVs can be isolated using magnetic beads coated with antibodies in a minimal volume of plasma [54].
Flushed 2-cell embryos were washed in HBS/BSA, transferred into the electroporation microchamber slides (VWR) in a minimal volume of medium and electroporated using an ECM®830 electroporator (VWR).
The residue was dissolved in a minimal volume of water and purified using ion-exchange-chromatography.
Two-photon microscopy is then used to photoexcite the benzophenone in a 1 micron3 (10,000 Å)3 volume as a minimal volume.
The final pellet was re-suspended in a minimal volume of the wash medium and used in the experiments.
If freeze-dried rinderpest vaccine is used as the standard antigen, it should be reconstituted in a minimal volume of PBS.
PCR products were processed using QIAquick PCR-Purification (QIAGEN) or MinElute PCR Purification Kits (QIAGEN), eluting DNA in a minimal volume of sterile water.
21 The mitochondrial pellet was washed twice and resuspended in a minimal volume of isolation medium and kept on ice until used.
Dry peptide films were dissolved in a minimal volume of 60 mM NaOH followed by dilution with deionized water and sonication for 1 min using a bath sonicator.
Adenovirus-mediated gene transfer was performed by adding a minimal volume of medium containing 2% FBS and adenovirus.
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