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Absorbance readings were taken at 450 nm using a microwell plate reader (Molecular Devices, Wokingham, UK).
Absorbance was read at 550 nm using a microwell plate reader.
Absorbance was measured at 405 nm using a microwell plate reader.
Cell densities were determined using optical density measurements at 750 nm (OD750) using a microwell plate reader (Infinite M200 PRO, Tecan Group Ltd., Männedorf, Switzerland).
The antigen-antibody reactions were measured by determining the optical density (O.D). at 410 nm using a microwell plate reader (MR-600, Dynatech Lab Inc .. To examine the ability of the cell line to form spheres, YES-2 and YES-2S cell lines were cultured in stem cell medium.
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Here, we present an innovative approach to culture Kaposi's sarcoma-associated herpesvirus (KSHV) infected human B cells in 3-D using a microwell array system.
The plates were then incubated at room temperature for one hour in 100 μL of streptavidin-HRP enzyme conjugate (1 : 1000), and the plates were read immediately using a spectrophotometer microwell plate reader at 450 nm.
Optical density (OD) was measured at 450 nm using an automated microwell plate reader.
Quantitative polymerase chain reaction (qPCR) was performed using Power SYBR Green PCR Master Mix Life Technologiess) on a microwell plate-based cycler platform (LightCycler 480 System, Roche) and the data were analyzed using the LightCycler 480 software v. 1.5 (Roche).
Eighty seven colonies were picked at random and grown at 30 °C in a microwell plate in selective media with xylose as the sole carbon source (SCX URA−).
The selectivity of the method was readily established by the vivid colorimetric responses shown by signaling experiments conducted in a microwell plate.
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