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Mad scientists of an earlier era were content with using a microtome and glass slides to examine odd cell structures through a dusty lens.
Sections of 5-μm thickness were taken using a microtome (Leica RM 2135).
Donor corneal stromas were cut to 120 200 μm thickness slices using a microtome and then decellularized.
The slices were decalcified, dehydrated, fixed in paraffin and cut in 4 μm slices using a microtome.
Adult heads were cut using a microtome.
Seven-µm cross-sections were cut using a microtome (Microm HM 315R, Heidelberg, Germany).
Four µm paraffin sections were then cut using a microtome and collected onto slides for staining.
Tissues were paraffin-embedded and sectioned sagitally using a microtome at a thickness of 5 µm.
Sections were cut at 50 µm using a microtome (Microm, Walldorf, Germany).
Paraffin-embedded cell blocks were sectioned at 4 µm thickness using a microtome (Leica, Germany).
Following this, the eyes were embedded in paraffin and dissected sagittally using a microtome into 5 µm sections.
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