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After dissolving the formazan crystals by adding dimethyl sulphoxide (Merck, Amsterdam, The Netherlands), plates were read immediately at 520 nm using a microtitre well spectrometer (Bio-Rad microplate reader, Bio-Rad laB.V.tories B.Veenendaaldaal, The Netherlands).
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The formazan was dissolved in 90 μl per well 1 N HCl, and the absorbance of the solution was read at 450 nm using a microtitre plate reader (Becton Dickinson, Franklin Lakes, NJ, USA).
Finally, absorption was determined at 620 nm using a microtitre plate reader (Multiskan, Finland).
The OD was determined using a microtitre plate reader.
A coloured end product is determined using a microtitre plate reader, measuring absorbance at 405 410 nm.
Hydrolysed pNA was detected using a microtitre plate reader at 405 nm.
The absorbance measurements were read using a microtitre plate reader (VERSAmax, Molecular Devices, Labotec).
Absorbance was calculated from the absorbance at 450 nm measured using a microtitre plate reader.
They were reconfirmed by using Microbact GNB 12A system (Oxoid, UK), a microtitre well-scaled chemical test.
The test was performed using a 96-well microtitre plate and optical density was read at 405 nm.
The release of free pNA was monitored colorimetrically at 405 nm for 1 h using a multi-well microtitre plate reader (Spectra MAX 340pc; Molecular Devices).
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