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Finally, absorption was determined at 620 nm using a microtitre plate reader (Multiskan, Finland).
The phytate content was estimated indirectly by using a microtitre plate assay evaluating the Pi absorbance at 820 nm, while the Cu, Fe, Mn, Ca, K, Mg, Na and Zn mineral contents were determined by ICP/OES.
Cells were incubated with MTT reagent (dimethylthiazol-2-yl-2,5-diphenyltetrazolium) for 3 hours at 37°C, solubilized in isopropanol:hydrochloric acid (24∶1) solution and the optical density (562 650 nm) was determined using a microtitre plate reader.
The OD was determined using a microtitre plate reader.
Hydrolysed pNA was detected using a microtitre plate reader at 405 nm.
The absorbance measurements were read using a microtitre plate reader (VERSAmax, Molecular Devices, Labotec).
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measures free DPD cross links in urine using competitive immunoassay in a microtitre plate format, using a monoclonal anti-D-Pyr antibody coated on the plate, to capture the DPD.
The mean optical density (OD) was used for quantification using a routine microtitre plate reader at a wavelength of 570 nm.
In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays.
Optical density (O.D) at 490 nm was determined using a ELISA microtitre plate reader.
Colour development, resulting from reduction of a redox dye (tetrazolium violet) added to the culture medium (Text S1), was used as a surrogate of metabolic activity, and was measured spectrophotometrically (λ = 600 nm) at 0, 8, 16, 24, 32, 40 and 48 h using a Thermomax microtitre plate reader (Molecular Devices, Wokingham, UK).
More suggestions(15)
using a witness plate
using a microchannel plate
using a microtiter plate
using a filter plate
using a beta plate
using a microtitre well
using a calibration plate
using a transwell plate
using a spectrophotometer plate
using a microwell plate
using a microtitration plate
using a microtitre protocol
using a titanium plate
using a ViewLux plate
using a multimode plate
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