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The absorbance was measured using a microtiter plate reader (SpectraMax 190, Molecular Device, USA) at a wavelength of 570 nm.
The ability of C. jejuni to form a biofilm in monoculture and mixed-microbial populations was measured in a laboratory assay using a microtiter plate screening assay.
The MIC of purified lipopeptide was evaluated by using a microtiter plate dilution assay.
The MIC of antimicrobial peptide was evaluated for different strains by using a microtiter plate dilution assay.
One hundred microliters of destaining solution was measured at 490 nm using a microtiter plate reader (iMark Microplate Reader S/N 12883 Biorad Pvt Ltd. India).
After 48 h, XTT and incorporated BrdU were measured colorimetrically using a microtiter plate reader (Bio-Rad Corp., Hercules, CA, USA) at a wavelength of 450 nm [42].
The reaction (in quadruplicate) was initiated by the addition of 30 μL purified enzyme solution (112.5 μg protein/mL) and p-nitroaniline release was monitored at 405 nm using a microtiter plate reader.
The amount of the formed blue formazan is proportional to the amount of viable cells[89], and the absorbance was measured at 492 nm using a microtiter plate reader (Tecan).
Microdilution plates were incubated at either 20 or 5°C and the optical density (OD) at 580 nm was recorded at specific time intervals using a microtiter plate reader/ spectrophotometer (Spectra MAX 190, Molecular Devices, CA, USA).
Subsequently the OD was measured at 570 nm using a microtiter plate reader (Model Sunrise, Tecan).
Vh luminescence and growth rate were measured in mini-batch cultures using a microtiter plate format.
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